An antibody was produced and used in an immunohistochemical assay to detect the location of RNA polymerase I in a thin section of a liver biopsy.The section was stained with the antibody that had been labeled with a fluorescent tag,then viewed under a fluorescent microscope.State where you would expect to find positive staining if the enzyme is present.

Question

Quizplus · Accepted Answer

RNA polymerase I is primarily involved in the transcription of ribosomal DNA, so it is expected to be present in the nucleolus, where ribosomal DNA is located. Therefore, positive staining is expected in the nucleolus.

An Antibody Was Produced and Used in an Immunohistochemical Assay