An experiment is planned to look at the transcription start site for the spoIID gene during sporulation.Which of the following techniques would yield the most information in this experiment?

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S1 mapping is a technique used to map the precise location of transcription start sites. It involves hybridizing labeled DNA probes to RNA transcripts, and then using the S1 nuclease to digest the single-stranded regions of the RNA, leaving only protected fragments that can be visualized on a denaturing gel. This technique is widely used for mapping the start sites of transcription in prokaryotes and eukaryotes, making it the best choice for this experiment. Southern analysis is used to detect specific DNA fragments by hybridizing them to a labeled DNA probe that is complementary to a specific sequence. Northern analysis is used to detect specific RNA molecules by hybridizing them to a labeled DNA probe that is complementary to a specific sequence. Run-on assays involve labeling newly synthesized RNA in a cell, isolating the labeled RNA, and hybridizing it to a DNA probe to determine which genes are actively being transcribed. DNAse footprinting involves using DNAse to cleave the DNA at random points, and then hybridizing the resulting fragments to a labeled DNA probe to determine which regions of the DNA are protected by bound proteins. None of these techniques are designed specifically for mapping transcription start sites.

An Experiment Is Planned to Look at the Transcription Start