Which of the following would most likely occur if an in vitro transcription assay is performed using a cell extract containing an alpha subunit without the C-terminal domain?

Question

Quizplus · Accepted Answer

The C-terminal domain of the alpha subunit is responsible for interaction with regulatory proteins and is important for promoter recognition and transcription initiation. Without the C-terminal domain, the polymerase complex would be loosely associated with the promoter, decreasing the efficiency of transcription initiation. Therefore, choice D is the best choice. Choices A, B, C, and E are unlikely to occur.

Which of the Following Would Most Likely Occur If an in Vitro