A 3000 bp circular DNA was treated with both HindIII and EcoRI restriction enzymes.EcoRI cuts at position 100.HindIII cuts at positions 500 and 2000.Which of the following represents the fragment that would be observed if the restricted DNA were analzyed using agarose gel electrophoresis?

Question

Quizplus · Accepted Answer

The HindIII enzyme will cut the circular DNA at positions 500 and 2000, creating two fragments of 1500 bp and 500 bp.

The EcoRI enzyme will cut the circular DNA at position 100, creating two fragments of 100 bp and 2900 bp.

Therefore, the resulting fragments are: 
- 1500 bp from HindIII 
- 500 bp from HindIII 
- 2900 bp from EcoRI

Combining the 1500 bp and 500 bp fragments from HindIII, we get a fragment of 2000 bp.

Therefore, the fragments observed in agarose gel electrophoresis would be 400 bp, 500 bp, and 1500 bp, which corresponds to Choice B.

A 3000 Bp Circular DNA Was Treated with Both HindIII