The G-less cassette assay is conducted using a promoter fused to a short DNA sequence and can be used to measure the transcription activity in a cell.

Question

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The G-less cassette assay is commonly used to measure the transcription activity in a cell by fusing a promoter to a short sequence of DNA that lacks the nucleotide guanine (G). This assay relies on the ability of RNA polymerase to transcribe through the G-less cassette and synthesize a complementary RNA molecule, which can be detected and quantified using PCR or other methods. Thus, the correct answer is A, true.

The G-Less Cassette Assay Is Conducted Using a Promoter Fused