A fragment restricted with EcoRI enzyme can be used for ligation into a plasmid that was restricted with BamHI because both the insert and the plasmid contain sticky ends.

Question

Quizplus · Accepted Answer

EcoRI and BamHI generate different sticky ends, so a fragment cut with EcoRI will not be directly compatible for ligation with a plasmid cut with BamHI unless adapters or further modification is used.

A Fragment Restricted with EcoRI Enzyme Can Be Used for Ligation