A Young Couple Has Had Regular,unprotected Sexual Intercourse for 4

A young couple has had regular,unprotected sexual intercourse for 4 years,without a pregnancy.They are seeking help at an assisted reproduction/IVF clinic for infertility.The husband's sperm count and sperm structure are normal.The wife undergoes a round of IVF.She must inject herself with FSH,which produces large antral follicles visible on ultrasonography.She is then given an LH injection,and her eggs are collected in a long needle by transvaginal approach 16 hours later.After egg retrieval,the eggs are examined.Unfortunately,the team decides not to go forward with fertilization.A reason for this decision could be the following observation in all of the eggs:

A) Expansion of the cumulus cells
B) Persistence of a zona pellucida
C) Presence of a germinal vesicle (nucleus)
D) Presence of the first polar body
E) Presence of a metaphase plate