Organisms that grow very slowly, are non-culturable, are present in very small numbers or are mixed with a number of other bacteria may still be identified using

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PCR is a highly sensitive technique that can amplify small amounts of DNA, making it useful in identifying slow-growing or non-culturable organisms. Additionally, it can target specific genes or regions of DNA for identification. Southern blotting and gas chromatography of fatty acids also have applications in bacterial identification, but may not be as effective in identifying slow-growing or non-culturable organisms. Replica plating is a technique used for screening bacterial colonies for specific characteristics, but is not as useful for identifying individual organisms.

Organisms That Grow Very Slowly, Are Non-Culturable, Are Present in Very