Put the following steps in the correct order needed to produce a recombinant vector containing a human gene insert. I. Introduce the recombinant plasmid into a bacterial cell.
II) Isolate the human gene and the vector DNA using restriction enzymes.
III) Ligate the DNA fragments to produce a recombinant plasmid.
IV) Grow bacterial cells on a medium containing a selective agent (such as an antibiotic).

Question

Quizplus · Accepted Answer

The correct order for producing a recombinant vector containing a human gene insert is to isolate the human gene and the vector DNA using restriction enzymes (II), ligate the DNA fragments to produce a recombinant plasmid (III), introduce the recombinant plasmid into a bacterial cell (I), and grow bacterial cells on a medium containing a selective agent (IV). Therefore, the correct choice is B as it follows the correct order.

Put the Following Steps in the Correct Order Needed to Produce