Which of the following would be an appropriate sequence of temperatures for PCR?

Question

Quizplus · Accepted Answer

The appropriate sequence of temperatures for PCR is as follows:
1. Denaturation at 94°C: This step melts the double-stranded DNA template and produces single-stranded DNA molecules.
2. Annealing at 55-65°C: This step allows the primers to anneal to the single-stranded template DNA.
3. Extension at 72°C: This step allows the Taq polymerase to extend the primers and synthesize new DNA strands.
Therefore, choice B with temperatures of 94°C, 65°C, and 72°C is the best choice for PCR.

Which of the Following Would Be an Appropriate Sequence of Temperatures