A light microscope with an objective lens numerical aperture of 0.65 is capable of allowing two objects 400 nm apart to be distinguished when using light with a wavelength of 420 nm.
Section 2.02

Question

Quizplus · Accepted Answer

This is true according to the resolution equation, which states that resolution is equal to 0.61 times the wavelength of light divided by the numerical aperture of the objective lens. Plugging in the values given, we get a resolution of approximately 407 nm, which is smaller than the distance of 400 nm between the objects. Therefore, they can be distinguished.

A Light Microscope with an Objective Lens Numerical Aperture of 0.65