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Suppose one restriction enzyme (enzyme A)makes two cuts in a linear DNA fragment and another restriction enzyme (enzyme B)makes one cut in that same DNA.The cuts are not in the same place,and none of the cut fragments are exactly the same size.The restriction digest is set up on an electrophoresis gel.
-How many bands should the gel pick up if only enzyme A is used in the restriction digest?
A) Two
B) Three
C) Four
D) Five
E) Six

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Quizplus · Accepted Answer

Enzyme A creates two cuts, so it will produce three fragments of varying sizes. These three fragments should be visible as three distinct bands on the gel after electrophoresis.

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