Two plasmids are used in a recombinant DNA procedure.Plasmid 67A carries a gene for resistance to the antibiotic ciprofloxacin,and plasmid 65B carries a gene for resistance to the antibiotic tetracycline.The plasmids are cut with HindIII restriction endonuclease,and then mixed with DNA ligase.In this procedure,what is the role of tetracycline?
A) It enhances transformation.
B) It is a selectable marker.
C) It is a vector.
D) It ligates the DNAs.
E) It provides color to the bacterial colonies.

Question

Quizplus · Accepted Answer

Tetracycline acts as a selectable marker, allowing for the identification and selection of bacterial colonies that have taken up the plasmid carrying the tetracycline resistance gene. Those colonies that have not taken up the plasmid will not survive in the presence of tetracycline.

Two Plasmids Are Used in a Recombinant DNA Procedure