Which plasmid would allow a researcher to visually select a bacterial colony that has a recombinant plasmid using white light? [Note: The plasmid also has selectable reporter gene for ampicillin resistance (amp^r)outside of the cloning site.] A) A plasmid with the gene for X-gal in the cloning site B) A plasmid with the gene for green fluorescent protein (GFP)outside of the cloning site C) A plasmid with the LacZ reporter gene outside of the cloning site D) A plasmid with the LacZ reporter gene in the cloning site E) A plasmid with the green fluorescent protein (GFP)within the cloning site

Question

Quizplus · Accepted Answer

A plasmid with the LacZ reporter gene in the cloning site allows for blue/white screening. When a gene is inserted into the LacZ site, it disrupts the gene, resulting in white colonies, indicating successful recombination.

Which Plasmid Would Allow a Researcher to Visually Select a Bacterial