If a 5'-to-3' strand of DNA has the sequence GTCTATGCATTA,what is the template DNA strand that would be used for transcription?
A) 5'-GTCTATGCATTA-3'
B) 5'-CAGATACGTAAT-3'
C) 3'-GTCTATGCATTA-5'
D) 3'-CAGATACGTAAT-5'
E) 5'-GUCUAUGCAUUA-3'

Question

Quizplus · Accepted Answer

During transcription, RNA polymerase reads the template strand of DNA in the 3' to 5' direction and synthesizes the complementary RNA strand in the 5' to 3' direction. Therefore, to determine the template strand of DNA, we need to reverse the direction of the given sequence and take the complementary nucleotides. The correct sequence for the template DNA strand would be 3'-CAGATACGTAAT-5', which corresponds to choice D.

If a 5'-To-3' Strand of DNA Has the Sequence GTCTATGCATTA,what