The whole-genome shotgun approach for collecting the data used to determine the nucleotide sequence of microbial genomes uses

Question

Quizplus · Accepted Answer

The whole-genome shotgun approach involves the use of powerful computers and specialized software to assemble the sequences obtained from automated sequencing of random fragments of genomic DNA. Therefore, choice D, which includes both automated sequencing and computer technology, is the best choice. Choice A, Maxam Gilbert sequencing of cloned restriction fragments, is an older method that is not commonly used today. Choice B, powerful computers and specialized software, is an important component of the whole-genome shotgun approach, but on its own is not sufficient to generate the data needed to determine nucleotide sequences. Choice C, automated sequencing of random fragments of genomic DNA, is a key step in the whole-genome shotgun approach, but the sequencing data must be analyzed and assembled using computer technology.

The Whole-Genome Shotgun Approach for Collecting the Data Used to Determine