Would a Mutation in an Enzyme That Changes the Shape

Would a mutation in an enzyme that changes the shape of its active site change the function of the enzyme?

A) No,because the enzyme would probably bind to the substrate anyway.
B) No,because another enzyme would make up for it.
C) No,because the shape of the active site has no effect on its activity.
D) Yes,because the active sites of all the enzymes have to match in order to work.
E) Yes,because the substrate has to fit specifically into the active site to undergo the reaction.