In 1964,Nirenberg and Leder used the triplet binding assay to determine specific codon assignments.A complex of which of the following components was trapped in the nitrocellulose filter?
A) ribosomes and DNA
B) free tRNAs
C) charged tRNA, RNA triplet, and ribosome
D) uncharged tRNAs and ribosomes
E) sense and antisense strands of DNA

Question

Quizplus · Accepted Answer

The triplet binding assay involved a mixture of charged tRNA, RNA triplets, and ribosomes. When the ribosome encountered a complementary RNA triplet, it would bind the corresponding charged tRNA, forming a complex that could be trapped on a nitrocellulose filter. Therefore, choice C is the correct answer. Choices A, B, D, and E are incorrect because they do not involve all three components of the triplet binding assay.

In 1964,Nirenberg and Leder Used the Triplet Binding Assay to Determine