The PCR reaction mixture does not include:
A)all four deoxynucleoside triphosphates.
B)DNA containing the sequence to be amplified.
C)DNA ligase.
D)heat-stable DNA polymerase.
E)oligonucleotide primer(s).

Question

Quizplus · Accepted Answer

The PCR reaction mixture includes all four deoxynucleoside triphosphates, DNA containing the sequence to be amplified, heat-stable DNA polymerase, and oligonucleotide primer(s). However, DNA ligase is not required in the PCR reaction as it is utilized in other DNA manipulation techniques such as DNA cloning.

The PCR Reaction Mixture Does Not Include