The technique known as two hybrid analysis for detecting interacting gene products depend on:
A) activation of DNA polymerase by the nearby binding of hybridizing protein complexes.
B) direct binding of a Gal4p activation domain to a DNA sequence in the promoter region.
C) having a promoter that responds directly to one of the two proteins whose interactions is being measured.
D) hybridization of DNA segments corresponding to the two genes being examined.
E) stimulation of transcription by interaction of two Gal4p domains via fused protein sequences.

Question

Quizplus · Accepted Answer

Two-hybrid analysis relies on the interaction between two Gal4p domains via fused protein sequences. When two proteins interact, the Gal4p domains are brought close together and can activate transcription of a reporter gene. Therefore, the correct answer is E, "stimulation of transcription by interaction of two Gal4p domains via fused protein sequences."

The Technique Known as Two Hybrid Analysis for Detecting Interacting