To calculate the turnover number of an enzyme,you need to know: A)the enzyme concentration. B)the initial velocity of the catalyzed reaction at [S] K_m. C)the initial velocity of the catalyzed reaction at low [S]. D)the K_m for the substrate. E)both A and B.

Question

To calculate the turnover number of an enzyme,you need to know: A)the enzyme concentration. B)the initial velocity of the catalyzed reaction at [S] >> K_m. C)the initial velocity of the catalyzed reaction at low [S]. D)the K_m for the substrate. E)both A and B.

Quizplus · Accepted Answer

The turnover number (k_cat) is calculated using the maximum reaction velocity (V_max) and the enzyme concentration. V_max is determined when substrate concentration [S] is much greater than K_m, which ensures the enzyme is saturated with substrate.

To Calculate the Turnover Number of an Enzyme,you Need to Know