Which of the following is least likely to result in protein denaturation?
A)Altering net charge by changing pH
B)Changing the salt concentration
C)Disruption of weak interactions by boiling
D)Exposure to detergents
E)Mixing with organic solvents such as acetone

Question

Quizplus · Accepted Answer

Changing the salt concentration is unlikely to result in protein denaturation because it primarily affects the strength of ionic interactions between charged amino acid residues, which are generally not essential for maintaining the protein's native conformation. Other choices can lead to disruption of more critical weak interactions (such as hydrogen bonds, hydrophobic interactions, and van der Waals forces), which can cause unfolding or aggregation of the protein.

Which of the Following Is Least Likely to Result in Protein