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The Following Reagents Are Often Used in Protein Chemistry

Question 64

Matching

The following reagents are often used in protein chemistry. Match the reagent with the purpose for which it is best suited. Some answers may be used more than once or not at all; more than one reagent may be suitable for a given purpose.

Premises:
breakage of disulfide (-S-S-)bonds
cleavage of peptide bonds on the carboxyl side of Met
determining the amino-terminal amino acid in a polypeptide
hydrolysis of peptide bonds on the carboxyl side of Lys and Arg
modification of sulfhydryl groups of Cys
determination of the amino acid sequence of a peptide
Responses:
reducing agent (dithiothreitol)
Sanger reagent (FDNB)
Edman reagent (phenylisothiocyanate)
chymotrypsin
trypsin
iodoacetamide
CNBr (cyanogen bromide)
performic acid

Correct Answer:

reducing agent (dithiothreitol)
Sanger reagent (FDNB)
Edman reagent (phenylisothiocyanate)
chymotrypsin
trypsin
iodoacetamide
CNBr (cyanogen bromide)
performic acid
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