How should vitreous washings be processed in the laboratory for recovery of bacterial pathogens?

Question

Quizplus · Accepted Answer

Passing the fluid through a 0.45 µL membrane filter will remove non-bacterial debris and allow for the recovery of bacteria on the filter, which can then be transferred to media for culture. This method is a common technique used for processing vitreous washings in the laboratory. Direct plating or dilution with saline may not provide optimal recovery of bacterial pathogens, while centrifugation may not effectively remove debris from the sample.

How Should Vitreous Washings Be Processed in the Laboratory for Recovery