In this level of the biofilm, the organisms slightly downregulate their metabolic activity, but they can still use nutrients and exchange genes.

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In the intermediate level of the biofilm, the organisms are still able to receive nutrients and exchange genes, but they slightly downregulate their metabolic activity. The outer layer of the biofilm is mostly inactive while the inner layer is completely inactive, and the substrata layer is where the biofilm attaches to a surface.

In This Level of the Biofilm, the Organisms Slightly Downregulate