How is the true Gram stain reaction of an anaerobe determined?

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Gram staining is the most common method for differentiating between Gram-positive and Gram-negative bacteria. However, some anaerobic bacteria may not stain correctly using the standard Gram staining procedure. To determine the true Gram stain reaction of an anaerobe, special potency disks can be used. These disks contain a high concentration of crystal violet, which helps to ensure that the bacteria are stained correctly.

How Is the True Gram Stain Reaction of an Anaerobe