A Microbiology Technologist Is Performing A -Lactamase Test on a Staphylococcus Isolated from a Hospitalized Patient
A microbiology technologist is performing a -lactamase test on a staphylococcus isolated from a hospitalized patient. The test was negative, so the technologist reported out in vitro antimicrobial susceptibility test results for -lactam antibiotics. After 2 days, the patient was not improving and the physician changed the patient's antibiotic to an aminoglycoside. The patient showed improvement immediately. Why was the -lactamase test on the staphylococci negative, when the organism produced -lactamase?
A) The test was a false negative-the organism really did produce -lactamase, but it did not react with the test.
B) Staphylococci must be exposed to the -lactam agent before it will produce a positive test result.
C) The test was a false positive-the organism reacted with the test, but it did not produce a -lactamase.
D) The staphylococci on the plate were more than 24 hours old and false-negative test results can occur when an "old" organism is used.
Correct Answer:
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