What chemical has been very successful in reducing carryover from PCR assays?

Question

Quizplus · Accepted Answer

Uracil-N-glycosylate is an enzyme that cleaves and removes any uracil present in the PCR reaction, which is introduced by dUTP instead of dTTP. This reduces carryover contamination from previous PCR reactions as the uracil-containing DNA is degraded by the enzyme during the subsequent PCR reaction. Taq DNA polymerase (A) is commonly used in PCR assays but does not directly reduce carryover contamination. Thymine (C) is one of the four nucleotides commonly used in PCR and does not have a specific role in reducing carryover contamination. Bromothymol blue (D) is a pH indicator and also has no specific role in reducing carryover contamination.

What Chemical Has Been Very Successful in Reducing Carryover from PCR