A newly sequenced gene is cloned and expressed in a bacterial cell.The bioinformatics databases suggest that this gene produces an enzyme that catalyzes the conversion of carbon dioxide to bicarbonate.After weeks of experiments,a scientist determined the enzyme has a completely different function.This is an example of_______ .
A)incorrect qPCR
B)incorrect BLAST parameters
C)incorrect annotation
D)incorrect enzyme experiments
E)incorrect sequencing

Question

Quizplus · Accepted Answer

This scenario describes incorrect annotation of the gene in the databases. The gene was annotated as encoding an enzyme that catalyzes the conversion of carbon dioxide to bicarbonate, but further experiments revealed a different function. This highlights the importance of rigorous experimental validation of bioinformatics predictions.

A Newly Sequenced Gene Is Cloned and Expressed in a Bacterial