Assume that a plasmid circular is 2800 base pairs in length and has restriction sites for EcoRI at the following locations: 400,700,1400,and 2600.Give the expected sizes of the restriction fragments following complete digestion with EcoRI.
A)200,400,700,1200
B)700,400,1400,2600
C)300,600,700,1200
D)300,700,2200
E)400,1200,1600

Question

Quizplus · Accepted Answer

The plasmid is cut at 400, 700, 1400, and 2600 base pairs, resulting in fragments of 300 (700-400), 700 (1400-700), 1200 (2600-1400), and 600 (2800-2600+400) base pairs.

Assume That a Plasmid Circular Is 2800 Base Pairs in Length