A PCR was designed to clone a DNA sequence of 1.5 kb.When the products were run on an agarose gel,there was a smear of bands ranging from 1.5 kb to 6 kb.Which of the following is a reason for this result?
A) one of the primers did not bind
B) the thermocycler malfunctioned
C) the polymerase overran the reverse primer
D) the polymerase denatured
Correct Answer:
Verified
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