Often Point Mutations Can Cause a Protein to Be Made

Often point mutations can cause a protein to be made that differs in just one amino acid.Which of the following changes is most likely to cause the greatest change in protein function?

A) a mutation that replaces an asparagine with a glutamine
B) a mutation that replaces a valine with an isoleucine
C) a mutation that replaces a proline with an aspartic acid
D) a mutation that replaces a glutamic acid with an aspartic acid
E) a mutation that replaces a serine with a cysteine