In 1964,Nirenberg and Leder used the triplet binding assay to determine specific codon assignments.A complex of which of the following components was trapped in the nitrocellulose filter?
A)charged tRNA,RNA triplet,and ribosome
B)ribosomes and DNA
C)sense and antisense strands of DNA
D)free tRNAs
E)uncharged tRNAs and ribosomes

Question

Quizplus · Accepted Answer

The triplet binding assay involves complex formation between a specific charged tRNA, an RNA triplet (representing a codon), and a ribosome. This complex is then trapped on a nitrocellulose filter, allowing for determination of specific codon assignments. Therefore, choice A is the best answer as it includes all three components necessary for the assay.

In 1964,Nirenberg and Leder Used the Triplet Binding Assay to Determine