According to Map 1.2: Native North American Cultures,what was among the most important factors in setting boundaries between ancient cultures?

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Map 1.2 shows that ancient Native North American cultures were separated by different environmental features such as mountains, rivers, and forests. These physical barriers acted as natural boundaries and contributed to the development of distinct cultural groups. While there were certainly differences in political systems, language, and religion, these were often influenced by and intertwined with the unique environments in which they existed.