DNA fragments must be denatured during the polymerase chain reaction due to which of the following?
A) Taq polymerase breaks down when heated
B) the repeated gene sequences are not present in double-stranded DNA
C) the primer must bind to a single-stranded template to synthesize double-stranded DNA
D) VNTRs have DNA sequences that vary in number

Question

Quizplus · Accepted Answer

In order for the polymerase chain reaction to occur, primers must bind to a single-stranded template to synthesize double-stranded DNA. Denaturation is necessary to separate the two strands of the double-stranded DNA in order for the primers to bind to a single-stranded template.

DNA Fragments Must Be Denatured During the Polymerase Chain Reaction