What is the substance required to cleave the vector DNA during recombinant DNA technology?
A) restriction enzymes
B) DNA ligase
C) plasmids
D) DNA polymerase
E) DNA helicase

Question

Quizplus · Accepted Answer

Restriction enzymes are used to cleave the vector DNA (usually a plasmid) and the source DNA (which contains the gene of interest) at specific recognition sites, allowing them to be joined together through complementary base pairing and ligation with DNA ligase. DNA polymerase and DNA helicase are not directly involved in the cleavage process.

What Is the Substance Required to Cleave the Vector DNA