Hydrodynamic focusing is used in automated cell-counting instruments to:
A) Prevent coincidence passage through the detection aperture
B) Increase the visibility of the cells passing through the aperture
C) Allow for maximal light absorbance through the cells passing through the aperture
D) Ensure adequate uptake of fluorescent staining by the cells

Question

Quizplus · Accepted Answer

Hydrodynamic focusing is a technique used in flow cytometry and automated cell-counting instruments to align cells so that they pass through the detection aperture one at a time. This prevents multiple cells from passing through the aperture simultaneously, which could lead to inaccurate counts and analyses. This process is known as preventing coincidence passage.

Hydrodynamic Focusing Is Used in Automated Cell-Counting Instruments To