Give the order (1^st to last)in which the following amino acids would be eluted with a buffer of pH = 4 from a column containing a cationic-exchange resin (Dowex 50): Arg (pI = 10.8),Ser (pI = 5.7),Asp (pI = 2.8)and His (pI = 7.6).

Question

Quizplus · Accepted Answer

At pH 4, amino acids with a pI below the buffer pH will be negatively charged and elute first, while those with a pI above the buffer pH will be positively charged and bind to the cationic-exchange resin, eluting later. Asp (pI = 2.8) is the most negatively charged, followed by Ser (pI = 5.7), then His (pI = 7.6), and finally Arg (pI = 10.8) is the most positively charged and elutes last.

Give the Order (1st to Last)in Which the Following Amino

Give the Order (1^st to Last)in Which the Following Amino