You Expose a Tube of Bacteria to Ultraviolet Radiation to Induce

You expose a tube of bacteria to ultraviolet radiation to induce mutations.You then spread the bacteria on a plate containing media with all known required nutrients and wait until separate colonies form.Finally,you use replicate plating to transfer a print of this plate to an additional sterile plate containing minimal media plus a known required nutrient for the bacteria.Based on this protocol,colonies growing on the plate with minimal media would include what type of cells?

A) only wild-type bacteria
B) only mutant bacteria
C) mutants that only require the supplied nutrient
D) Both wild-type and all mutants
E) wild-type bacteria and the mutants that only require the supplied nutrient