A biochemist wants to control the initial substrate-level phosphorylation that occurs in the tracheal cells of grasshoppers once glucose has crossed the plasma membrane.He has access to the following inhibitors: Rotenone - an electron transport chain inhibitor,Oligomycin - an ATP synthase inhibitor,and TLN-232,an inhibitor of glycolysis.Which inhibitor should he use to slow down initial substrate-level phosphorylation that occurs once glucose has crossed the plasma membrane?

Question

Quizplus · Accepted Answer

TLN-232 is an inhibitor of glycolysis, which is the pathway that leads to substrate-level phosphorylation. By inhibiting glycolysis, TLN-232 would prevent the formation of the substrates necessary for substrate-level phosphorylation. Rotenone and Oligomycin would not be effective in preventing substrate-level phosphorylation as they target later steps in cellular respiration (electron transport chain and ATP synthase, respectively) and not the initial steps of glycolysis.

A Biochemist Wants to Control the Initial Substrate-Level Phosphorylation That