Your research project involves characterizing the expression pattern of a nuclear pore protein.After using immunohistochemistry to fluorescently label the protein,you decide that you would like to capture the data as a 3-D image.Which microscope should you use?
A)phase-contrast microscope
B)fluorescence microscope
C)confocal microscope
D)scanning electron microscope

Question

Quizplus · Accepted Answer

Confocal microscope is the best choice for capturing a 3-D image of a fluorescently labeled nuclear pore protein because it allows for optical sectioning of the specimen and can eliminate out-of-focus information that can lead to distortion or blurring of the image. This results in a clearer and more precise image of the protein's expression pattern, making it easier to analyze and interpret the data. Phase-contrast microscope, fluorescence microscope, and scanning electron microscope do not offer the same level of optical sectioning or 3-D imaging capabilities as a confocal microscope.

Your Research Project Involves Characterizing the Expression Pattern of a Nuclear