In the cloning of a gene by PCR,DNA is first made from mRNA by using which enzyme?

Question

Quizplus · Accepted Answer

In the cloning of a gene by PCR, DNA is made from mRNA by using reverse transcriptase, which synthesizes a complementary DNA strand from an RNA template via reverse transcription. DNA polymerase is used in PCR for the amplification of the DNA fragment. RNA polymerase is involved in the transcription of DNA to mRNA. Aminoacyl-tRNA synthetase is involved in the charging of tRNAs with their respective amino acids during translation.

In the Cloning of a Gene by PCR,DNA Is First