According to Map 3.1: Chesapeake Colonies in the Seventeenth Century, what river led most directly to the earliest established Chesapeake settlements?

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The James River led most directly to the earliest established Chesapeake settlements, including Jamestown, which was the first permanent English settlement in North America. The Potomac and Rappahannock Rivers were also important waterways for trade and transportation in the Chesapeake colonies, but they were not as directly linked to the earliest settlements as the James River was. The Susquehanna River was located further north and did not have significant settlements in the Chesapeake region.