DNA sequencing gives a readout of 5' AATCGGTTG 3' based on the color-labeled nucleotides detected after gel electrophoresis. What is the sequence of the template strand?
A)5' AATCGGTTG 3'
B)5' CAACCGATT 3'
C)5' TTAGCCAAC 3'
D)5' GTTGGCTAA 3'
E)More information is required to determine the sequence of the template strand.

Question

​DNA sequencing gives a readout of 5' AATCGGTTG 3' based on the color-labeled nucleotides detected after gel electrophoresis. What is the sequence of the template strand?
A)​5' AATCGGTTG 3'
B)​5' CAACCGATT 3'
C)​5' TTAGCCAAC 3'
D)​5' GTTGGCTAA 3'
E)​More information is required to determine the sequence of the template strand.

Quizplus · Accepted Answer

The template strand is the strand of DNA that is complementary to the sequencing readout. The sequencing readout is 5' AATCGGTTG 3', so the template strand must be 5' CAACCGATT 3'.

​DNA Sequencing Gives a Readout of 5' AATCGGTTG 3' Based

DNA Sequencing Gives a Readout of 5' AATCGGTTG 3' Based