Kawakubo's group created a glycoprotein with a terminal NAG i.e., a protein with NAG attached to its end). Their hypothesis is that the terminal NAG-and not the protein component-is responsible for the damage to the cell wall in H. pylori. What would be the most appropriate control for testing this hypothesis?
A) Grow H. pylori in a test tube with glycoprotein that has its terminal NAG removed.
B) Destroy the H. pylori by exposing them to a hypotonic solution. Then add the glycoprotein and observe.
C) Expose other species of bacteria to the glycoprotein.
D) Grow H. pylori in a test tube in vitro) with no glycoprotein.

Question

Quizplus · Accepted Answer

To test whether the terminal NAG is responsible for the damage to the cell wall in H. pylori, a control should be used in which the glycoprotein has its terminal NAG removed. This will allow for the comparison of the effects of the glycoprotein with and without the NAG component on H. pylori cell walls. Option B is not appropriate as it does not address the hypothesis being tested. Option C is not relevant as it does not involve H. pylori, which is the focus of the hypothesis. Option D is not appropriate as it does not allow for the comparison of the effects of the glycoprotein with and without the NAG component.

Kawakubo's Group Created a Glycoprotein with a Terminal NAG I