The Microsporidian Brachiola Gambiae Parasitizes the Mosquito Anopheles Gambiae

The microsporidian Brachiola gambiae parasitizes the mosquito Anopheles gambiae. Adult female mosquitoes must take blood meals for their eggs to develop, and it is while they take blood that they transmit malarial parasites to humans. Male mosquitoes drink flower nectar. If humans are to safely and effectively use Brachiola gambiae as
A biological control to reduce human deaths from malaria, then how many of the following statements should be
1) Brachiola should kill the mosquitoes before the malarial parasite they carry reaches maturity.
2) The microsporidian should not be harmful to other insects.
3) Microsporidians should infect mosquito larvae, rather than mosquito adults.
4) The subsequent decline in anopheline mosquitoes should not significantly disrupt human food resources or food webs.
5) Brachiola must be harmful to male mosquitoes but not to female mosquitoes.

A) 2 and 5
B) 3 and 4
C) 2, 3, 5
D) 1, 2, 3, and 4
E) 1, 2, and 4