What is the right progression of steps for a direct- sequencing experiment?
A) Lyse cells, insert genes into plasmid, purify DNA, and amplify genes by PCR.
B) Purify DNA, amplify genes by PCR, lyse cells, and insert genes into plasmid.
C) Lyse cells, insert genes into plasmid, amplify genes by PCR, and purify DNA.
D) Lyse cells, purify DNA, amplify genes by PCR, and insert genes into plasmids.

Question

Quizplus · Accepted Answer

The correct progression involves lysing cells to release DNA, purifying the DNA, amplifying specific genes by PCR, and then inserting the amplified genes into plasmids if needed for further analysis or cloning.

What Is the Right Progression of Steps for a Direct-