What information is critical to the success of PCR itself?
A) The complete DNA sequence of the DNA to be amplified must be known.
B) The sequence of restriction enzyme recognition sites in the DNA to be amplified and in the plasmid where the amplified DNA fragment will be cloned must be known.
C) The DNA sequence of the ends of the DNA to be amplified must be known.
D) The sequence of restriction enzyme recognition sites in the DNA to be amplified must be known.

Question

Quizplus · Accepted Answer

For PCR to be successful, it is essential to know the DNA sequence of the ends of the DNA to be amplified. This information is necessary to design the primers that will anneal to the DNA and initiate the amplification process.

What Information Is Critical to the Success of PCR Itself