Beadle discovered that metabolic pathways are studied most effectively using which of the following techniques?
A) gene amplification
B) adding energy in the form of ATP
C) adding additional intermediates of the metabolic pathway
D) creating single gene mutations resulting in nonfunctional enzymes specific to a metabolic pathway

Question

Quizplus · Accepted Answer

Creating single gene mutations resulting in nonfunctional enzymes specific to a metabolic pathway allows for the study of the effects of specific enzyme deficiencies on the overall pathway. This technique is known as genetic analysis and was used by Beadle to study metabolic pathways. Choice A is incorrect because gene amplification is used to increase the expression of a gene of interest, not to study metabolic pathways. Choice B is incorrect because adding energy in the form of ATP would not necessarily provide any insight into the workings of a metabolic pathway. Choice C is incorrect because adding intermediates would only give information about the intermediates that were added, not the overall pathway.

Beadle Discovered That Metabolic Pathways Are Studied Most Effectively Using