Figure 9.5 -In Figure 9.5, the marker genes used for selecting recombinant DNA are A) lacZ and ori. B) amp^Rand ori. C) ori. D) amp^Rand lacZ. E) HindIII, BamHI, and EcoRI.

Question

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The marker genes used for selecting recombinant DNA in Figure 9.5 are amp^Rand lacZ. The use of lactose as a carbon source in the presence of X-gal (a substrate of the β-galactosidase enzyme encoded by lacZ) allows identification of cells containing plasmids with inserts (white colonies) versus cells containing empty vector (blue colonies). The ampR gene provides resistance to ampicillin (amp^R), allowing for selection of cells that have taken up the plasmid vector.

Figure 9.5 -In Figure 9.5, the Marker Genes Used for Selecting Recombinant